Fig 1: Expression levels of the PDK family in cancers. The figure (a–d) showing upregulated (red) or downregulated (blue) mRNA expression of PDK family of different cancers compared with the corresponding normal tissues in the TCGA and GTEx database. * p < 0.05, ** p < 0.01, *** p < 0.001. PDK4, pyruvate dehydrogenase kinase; TCGA, The Cancer Genome Atlas; GTEx, The Genotype-Tissue Expression Project.
Fig 2: Effect of PDK4 on the tumor cell growth. (a,b) Growth curves indicating that suppression of PDK4 inhibited HGC27 cell proliferation and overexpression of PDK4 promoted AGS cell proliferation. (c,d) The tumor colony formation assay indicating that suppression of PDK4 inhibited HGC27 cell proliferation and overexpression of PDK4 promoted AGS cell proliferation. Data are displayed as means ± SD. ** p < 0.01, *** p < 0.001.
Fig 3: FAM210B knockdown cells show decreased PDK4 expression. (a) Heat map of the top 25 differentially regulated genes in siFAM210B and negative control A549 cells. (b) Quantitative PCR analyses of the relative expression of the indicated genes in siFAM210B normalized to negative control SKOV3 cells. (c) Immunoblotting for PDK4 in the indicated treated SKOV3 cells. (d) Schematically depicted PDK function in SKOV3 cell metabolism. (e) Western blotting analysis of the phosphorylation levels of PDH-E1a in the indicated treated SKOV3 cells. (f) Normalized OCR and (g) normalized ECAR in siPDK4 (n=6) and negative control SKOV3 cells (n=8 wells)
Fig 4: – Adropin reduces Pdk4 expression in rat neonatal cardiomyocytes. (A-D) Adropin treatment significantly reduced Pdk4 gene expression in rat neonatal cardiomyocytes without affecting fatty acid oxidation pathway enzymes (Ppargc1a, Cd36, and Cpt1a) Con = control, Adr = Adropin. N = 3, * = P < 0.05 (Student's T-Test).
Fig 5: Identification of DEGs and enrichment analyses of PDK4 in GC. (a) Volcano plots of the DEGs. (b) Bubble chart of GO results displaying DEGs in low- and high- PDK4 expression samples. (c) GSEA analysis of the DEGs between low- and high- NOD1 expression groups. DEGs, differentially expressed genes. GO, gene ontology. GSEA, gene set enrichment analysis.
Supplier Page from Abcam for Anti-PDK4 antibody [1C2BG5]